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px459 addgene 62988 vector  (Addgene inc)


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    Addgene inc px459 addgene 62988 vector
    Px459 Addgene 62988 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+addgene+62988+vector/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/bio_rxiv__64898__2026__03__10__710821-168-5-6
    Average 96 stars, based on 2935 article reviews
    px459 addgene 62988 vector - by Bioz Stars, 2026-10
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    Purification:

    Article Title: Acute Degradation of Pumilio Proteins Uncovers a Biphasic Post-transcriptional Regulatory Hierarchy Controlling Embryonic Stem Cell Fate Decisions
    Article Snippet: .. The PX458 (addgene #48138) or PX459 (addgene #62988) vector was linearized with BbsI-HF digestion and gel purified. ..



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    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: G3BP1 is a tunable switch that triggers phase separation to assemble stress granules

    doi: 10.1016/j.cell.2020.03.046

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cytoscape version 3.7.1 ( Shannon et al., 2003 ) was used for the visualization of this network in . . CRISPR-Cas9-Mediated Knockout Cells gRNA for each gene was designed through http://crispr.mit.edu/ and synthesized DNA oligos were ligated into BbsI- (NEB) digested px459 vectors (Addgene, 62988) ( Ran et al., 2013 ).

    Techniques: Virus, Recombinant, Protease Inhibitor, Electron Microscopy, Lysis, Extraction, Staining, Isolation, Mutagenesis, Sequencing, Plasmid Preparation, Software, Imaging